Volume 3,Issue 9
疏肝和胃方含药血清对食管上皮细胞损伤的干预作用
目的：探讨疏肝和胃方含药血清对GERD 食管上皮细胞损伤的干预作用。方法：实验分四组，除正常组外，其余三组予胰蛋白酶刺激HEEC 构建GERD 体外细胞模型。正常组和模型组予生理盐水含药血清孵育，中药组予疏肝和胃方含药血清孵育，西药组予雷贝拉唑肠溶胶囊含药血清孵育。24h 后通过ELISA 方法检测IL-8、IL-1β 和PAF，WB 检测PAR-2、Claudin1和Claudin4。所得结果采用SPSS26.0 统计分析。结果：与正常组相比，模型组PAR-2、IL-8、IL-1β 和PAF 显著增加（P<0.05），Claudin1和Claudin4显著降低（P<0.05）；与模型组相比，中西药组PAR-2、IL-8和PAF 均显著降低（P<0.05），中药组IL-1β 显著降低（P<0.05），西药组IL-1β 呈降低趋势（P>0.05），且中药组表达均低于西药组，其中中西药组IL-8和PAF 表达有统计学差异（P<0.05），中西药组Claudin1 显著升高（P<0.05），中药组Claudin4 显著升高（P<0.05），西药组Claudin4 呈升高趋势（P>0.05），且中药组表达均显著高于西药组（P<0.05）。结论： 疏肝和胃方可以通过降低PAR-2、IL-8、 IL-1β 和PAF 的表达，增加Claudin1和Claudin4蛋白的表达，改善食管黏膜炎性损伤，有效的治疗GERD，且疗效优于雷贝拉唑。
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