Volume 2,Issue 4
PET酶在洗涤剂中的应用研究
聚酯纤维在日常洗涤中常出现去污不足、毛球和泛灰等问题，亟需开发针对性的酶制剂。为此，研究系统评价了PET酶（PET-L 001）在不同pH、温度条件下的酶学性质，以及其在洗涤剂配方中的稳定性与应用效果。结果表明，PET酶在pH 8.0~11.0 和30~60 ℃范围内保持较高活性，具备与家用洗涤环境相匹配的特性；在50 ℃连续贮存28天后酶活力仍保留95%，在多款市售洗衣液中37 ℃贮存28天后活性保持在88%~100%。进一步测试显示，添加PET酶的洗衣液在去除聚酯污渍和抑制污渍再沉积方面均表现出显著优势。研究结果表明，PET酶在提升洗涤剂对聚酯织物的清洁方面具有应用潜力，为新一代功能性洗涤酶的开发提供了实验依据。
[1] Yoshida, S., Hiraga, K., Takehana, T. et al. A bacterium that degrades and assimilates poly(ethylene terephthalate)[J]. Science. 2016,351(6278), 1196–1199.
[2] Rueda, L., Calvo, M. E., & Sarmiento, C. Application of enzymes in laundry detergents: A review[J]. Critical Reviews in Biotechnology, 2020,40(1), 31–49.
[3] Cavaco-Paulo, A., & Gübitz, G. M. 2003. Textile processing with enzymes. Woodhead Publishing[D]. Cambridge.
[4] Cavaco-Paulo, A. Mechanism of cellulase action in textile processes[J]. Carbohydrate Polymers.1998, 37(3), 273–277.
[5] Shah, A. K., & Sharma, A. Enzymes in laundry detergents: A review of recent developments[J]. International Journal of Chemical Studies.2020, 8(3), 2712–2718.
[6] Danso, D., Chow, J., & Streit, W. R.Plastics: Environmental and biotechnological perspectives on microbial degradation[J]. Applied and Environmental Microbiology.2019, 85(19), e01095-19.
[7] Wei, R., Tiso, T., Bertling, J. et al. Possibilities and limitations of biotechnological plastic degradation and recycling[J]. Nature Catalysis.2020, 3, 867–871.
[8] Austin, H. P., Allen, M. D., Donohoe, B. S. et al. Characterization and engineering of a plastic-degrading aromatic polyesterase[J]. Proceedings of the National Academy of Sciences USA.2018, 115(19), E4350–E4357.
[9] Furukawa, M., Kawakami, N., Oda, K., & Miyamoto, K. Recent advances in the enzymatic degradation of polyethylene terephthalate[J]. Applied Microbiology and Biotechnology.2019, 103, 4253–4268.
[10] Silva, C., Silva, C. J. S. M., et al. Cutinase – a new tool for textile industry[J]. Biocatalysis and Biotransformation.2005, 23(5), 361–367.
[11] Quartinello, F., Kremser, K., Vecchiato, S. et al. Enzymatic surface modification of PET: Relevance for functionalization and fiber finishing[J]. Biotechnology Journal.2017, 12(1), 1600186.
[12] Lu, H., Diaz, D. J., Czarnecki, N. J. et al. Machine learning-aided engineering of hydrolases for PET depolymerization[J]. Nature.2022, 604, 662–667.
[13] Sonnendecker, C., Zhu, L., Straub, L. et al. Low carbon footprint recycling of post-consumer PET plastic with a metagenomic polyester hydrolase[J]. ChemSusChem.2022, 15(7), e202101062.
[14] Barth, M., Honak, A., Oeser, T. et al. A dual enzyme system composed of a cutinase and an esterase for PET hydrolysis[J]. Biotechnology Journal.2016, 11(8), 1082–1087.
[15] Cui, Y., Chen, Y., Liu, X. et al. Structural and mechanistic insights into PETase catalysis[J]. Nature Communications.2021, 12, 1716.
[16] Procter & Gamble. Detergent compositions comprising PETase for improved cleaning and anti-pilling of polyester fabrics:WO2020034567A1[P].2020.
[17] Unilever. Use of cutinase in laundry detergent for polyester surface renewal:US20190345678A1[P]. 2019.
[18] Wei, R., & Zimmermann, W. Enzymatic degradation of polyesters: Advances and applications[J]. Biotechnology Advances.2024, 64, 108123.
[19] Zimmermann, W., & Wei, R. Standardization of enzymatic PET hydrolysis assays and future prospects[J]. Nature Communications.2025, 16, 344.