表面活性剂组合温和性的体外和在体研究
本研究通过建立体外人角质细胞模型，对6个单一表面活性剂的细胞活力影响进行评估。之后将两性/非离子表活和甲基椰油酰基牛磺酸钠-SMCT进行两元、三元复配并检测体外IC50值和炎症因子IL-1α水平。结果表明，在单一表活中，非离子表活尤其是海藻糖酯THL的IC50值最高、细胞毒性最低。在表活复配体系中，基于阴离子/两性/非离子表活复配的三元体系产生的IL-1α水平低于阴离子/两性表活复配的二元体系，且THL显示出比烷基糖苷APG在降低炎症因子方面更好的效果。最后，我们通过单次封闭式斑贴实验对复配体系进行了经皮水分散失率的测试，以及红斑反应评价。并综合分析了复配体系对皮肤脂质屏障的影响和引起的细胞炎症反应，多个因素作用下最终导致皮肤产生红斑等不良反应。
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